Figure 5: Flow cytometry analysis of integrin expression in HepaRG cells following APAP treatment for 24 hours. | Scientific Reports

Figure 5: Flow cytometry analysis of integrin expression in HepaRG cells following APAP treatment for 24 hours.

From: Low-dose acetaminophen induces early disruption of cell-cell tight junctions in human hepatic cells and mouse liver

Figure 5

Histograms representing relative expression of integrins (CD49a-f) and CD29 in HepaRG cells following challenge with different concentrations of APAP. Though the expression of most integrins was modulated over the period of exposure to APAP, only modulation of CD49c was directly associated with increasing APAP concentration. Untreated HepaRG cells contained high and low CD49c expressing populations. 24 hours after treatment, increasing APAP concentration was directly associated with increased percentage of cells in the low expressing population, and a reciprocal decrease in the high expressing population. The level of expression (MFI) was unchanged in the CD49c low population, whereas the level of expression by cells in the high population increased directly with APAP concentration. Detailed flow cytometric data is shown in Supplementary Table 2.

Back to article page