Table 2 Stability ranking of the candidate reference genes using five algorithms in different developmental stages of B. belcheri.

From: Selection of reliable reference genes for normalization of quantitative RT-PCR from different developmental stages and tissues in amphioxus

Gene

RefFinder

geNorm

NormFinder

BestKeeper

deltaCt

Rank

GM

Rank

SV

Rank

SV

Rank

SV

Rank

SV

S20

1

1.72

3

0.85

2

0.72

1

0.51

2

1.84

18 S

2

2.23

1

0.76

3

0.76

2

0.55

5

1.89

EF1A

3

2.74

7

1.49

1

0.64

7

1.71

1

1.82

CYC

4

3.11

1

0.76

5

0.84

3

0.63

4

1.88

G6PDH

5

4.79

4

0.95

9

1.03

4

0.67

3

1.86

UBC

6

5.92

6

1.29

6

0.92

6

1.28

8

2.04

L3

7

6.22

8

1.63

4

0.79

9

1.82

6

1.94

TBP

8

7.04

5

1.15

10

1.07

5

0.84

10

2.12

TUB

9

8.67

9

1.71

7

0.95

10

1.86

7

2.03

GAPDH

10

9.41

10

1.76

8

1.02

8

1.74

9

2.10

HPRT

11

11.37

11

1.85

12

1.46

11

1.89

12

2.59

ACT

12

12.26

12

1.99

11

1.41

12

2.59

11

2.54

L13

13

12.93

13

2.03

13

1.71

13

2.87

13

2.84

  1. Parameters of ranking were showed by using GM (geometric mean); SV, stability value. If no additional declarations, these abbreviations indicate the same means as above described in other tables.
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