Table 5 Stability ranking of the candidate reference genes using five algorithms in gill of B. belcheri challenged with LPS.
Gene | RefFinder | geNorm | NormFinder | BestKeeper | deltaCt | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Rank | GM | Rank | SV | Rank | SV | Rank | SV | Rank | SV | |
S20 | 1 | 1.21 | 1 | 0.51 | 1 | 0.30 | 7 | 1.04 | 1 | 1.40 |
EF1A | 2 | 2.95 | 3 | 0.78 | 2 | 0.41 | 8 | 1.10 | 3 | 1.44 |
UBC | 3 | 3.84 | 1 | 0.51 | 3 | 0.45 | 10 | 1.20 | 2 | 1.44 |
ACT | 4 | 3.91 | 5 | 0.97 | 4 | 0.49 | 2 | 0.64 | 5 | 1.51 |
TUB | 5 | 4.98 | 4 | 0.91 | 6 | 0.56 | 6 | 1.03 | 4 | 1.49 |
18 S | 6 | 5.61 | 10 | 1.31 | 10 | 0.99 | 1 | 0.52 | 11 | 1.86 |
HPRT | 7 | 6.05 | 7 | 1.11 | 7 | 0.84 | 4 | 0.94 | 7 | 1.72 |
L13 | 8 | 6.67 | 6 | 1.02 | 5 | 0.52 | 9 | 1.16 | 6 | 1.53 |
G6PDH | 9 | 6.77 | 9 | 1.27 | 9 | 0.90 | 3 | 0.72 | 8 | 1.79 |
TBP | 10 | 8.61 | 8 | 1.20 | 11 | 1.02 | 5 | 0.96 | 10 | 1.84 |
GAPDH | 11 | 9.85 | 11 | 1.38 | 8 | 0.90 | 11 | 1.67 | 9 | 1.81 |
CYC | 12 | 11.83 | 12 | 1.59 | 12 | 1.68 | 12 | 1.96 | 12 | 2.71 |
L3 | 13 | 12.74 | 13 | 1.80 | 13 | 1.91 | 13 | 2.49 | 13 | 2.97 |
