Figure 3

α-SMA expression in fibroblasts and myofibroblasts with or without treatment by di-rhamnolipid.
(a) Immunofluorescence staining of α-SMA (green). DAPI was used for nuclear counterstaining (blue). Scale bars = 2 μm. (b) α-SMA expression by Western blotting. (c) Data digitized from images of Western blotting by Image Pro Plus 6.0. Fib = fibroblast, Myofib = myofibroblast, R10, R20 and R30 = di-rhamnolipid at concentration of 10, 20 and 30 mg/L.