Figure 3

UC-MSCs impair the allostimulatory capacity of monocyte-derived cells.
After a 7-day incubation, monocytes were collected and cultured with PBLs labeled with CFSE for 5 days. The cells were the collected, and the proliferation of CD3+ T cells was analyzed by FACS (A,B). (A) One representative experiment of three is shown. (B) The data are the proliferation numbers of CD3+ cells and the means ± SEMs of three different healthy persons. The ability of CD4+ T cells to produce IFNγ (C), IL2 (D), and TNFa (E) were tested by FACS. The data are shown as the means ± SEMs of three different healthy persons.