Figure 5
The role of HGF, IL6 and IL10 in the UC-MSC-mediated inhibition of monocyte differentiation.
After a 5-day incubation, monocytes were collected and analyzed by FACS. The CD14 and CD1a expression in monocytes cultured alone (Control), with IL6, IL10, or HGF, or cocultured with UC-MSCs in a transwell co-culture system in the absence (+MSC (transwell)) or presence of anti-IL6, anti-IL10, or anti-HGF was determined (A,B). The data indicate the means ± SEMs of three independent experiments. After a 7-day incubation, monocytes cultured in the indicated conditions were collected to determine the mRNA levels of IL10 (C,D). The data are shown as the means ± SEMs of three independent experiments.
