Figure 1 | Scientific Reports

Figure 1

From: Autophagy is required for PDAC glutamine metabolism

Figure 1

Glutamine, but not glucose, deprivation induces autophagy.

(A) PDAC cells were plated in complete medium, which was replaced the following day with glucose or glutamine-free medium and then incubated for another 24 h. Cell lysates were immunoblotted for LC3 and p62. (B) 8988 T cells were cultured in complete or glutamine free medium with or without CQ (10 μM) for 24 h and immunoblotted for LC3. (C) 8988 T cells were infected with a lentivirus expressing GFP-LC3, grown in complete, glucose-free, or glutamine-free medium for 24 h and analyzed for LC3 dots. (D) PDAC cells were treated with 2DG (10 mM) or BPTES (10 μM) for 24 h and immunoblotted for LC3. (E) Effect of GLS1 knockdown on LC3 levels in 8988 T cells expressing a control shRNA (shGFP) or glutaminase 1 shRNAs (shGLS1s).

Back to article page