Table 1 Kinetic parameters of WT EcAhpC and its mutants.

From: Transition steps in peroxide reduction and a molecular switch for peroxide robustness of prokaryotic peroxiredoxins

EcAhpC

kcat ± SD (s−1)

Km ± SD (μM)

kcat/Km (M−1s−1)

WT EcAhpC

0.090 ± 0.001

1.44 ± 0.1

6.2 × 104

EcAhpC1–172

0.082 ± 0.004

9.45 ± 0.5

8.6 × 103

EcAhpCI187G

0.089 ± 0.003

8.26 ± 0.4

1.0 × 104

EcAhpCS86A,T88A

0.089 ± 0.004

9.74 ± 0.5

9.0 × 103

EcAhpC1-186-YFSKHN

0.082 ± 0.001

1.77 ± 0.1

4.6 × 104

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