Figure 18

Western blot assay of apoptosis related protein levels in Hep-G2 cells treatmented with complexes 1 (8 μM), 2 (15 μM), 3 (5 μM), 4 (18 μM), 5 (28 μM) and 6 (14 μM) for 24 h, respectively.
(A,C) Apoptosis related proteins protein levels in Hep-G2 cells were analyzed by western blot. (B,D) The whole-cell extracts were prepared and analyzed by Western blot analysis using antibodies against apoptosis related proteins. The same blots were stripped and reprobed with a β-actin antibody to show equal protein loading. Western blotting bands from three independent measurements were quantified with Image J. in (B,D).