Table 4 Abbreviations, DNA oligomers and cell lines of used in this work.
G4 | G-quadruplex |
|---|---|
FID | fluorescent intercalator displacement |
TO | thiazole orange |
SD | standard deviation |
Tm | melting temperature |
FRET | fluorescence resonance energy transfer |
TBS | pH 7.35, 10 mM Tris-KCl-HCl buffer solution, containing 100 mM KCl |
MTT | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
PI | propidium iodide |
TAMRA | 6-carboxytetramethylrhodamine |
PBS | phosphate buffered saline |
TRF | the duplex TTAGGG repeat-binding factors (such as TRF1 and TRF2) |
FAM | 6-carboxyfluorescein |
hTERT | human telomerase reverse transcriptase |
53BP1 | p53 binding protein 1 |
RT-PCR | reverse transcription-polymerase chain reaction |
Pu27 | 5′-TGGGGAGGGTGGGGAGGGTGGGGAAGG-3′ |
c-kit-2 | 5′-CGGGCGGGCGCTAGGGAGGGT-3′ |
c-kit-1 | 5′-CGGGCGGGCACGAGGGAGGGT-3′ |
HTG21 | 5′-GGGTTAGGGTTAGGGTTAGGG-3′ |
Pu39 | 5′-AGGGGCGGGCGCGGGAGGAAGGGGGCGGGAGCGGGGCTG-3′ |
ds26 | 5′-CAATCGGATCGAATTCGATCCGATTG-3′ |
Pu22 | 5′-TGAGGGTGGGTAGGGTGGGTAA-3′ |
F21T | 5′-FAM-GGGCTAGGGCTAGGGCTAGGG-TAMRA-3′ |
FMidG4T | 5′-FAM-CGGGCGCGGGAGGAAGGGGGCGGGAGC-TAMRA-3′ |
FPu18T | 5′-FAM-AGGGTGGGGAGGGTGGGG-TAMRA-3′ |
H20M | 5′-GCCAGTTCTTGAATGTAGAG-3′ |
F32T | 5′-FAM-CCGCATCTCTACATTCAAGAACTGGCATGCGG-TAMRA-3′ |
bcl-2 | Cx: 5′-GAGGATTGTGGCCTTCTTTG-3′ |
Ts: 5′-GCCGGTTCAGGTACTCAGTC-3′ | |
hTERT | Cx: 5′-CATCCACATAGAGGCCACCACGT-3′ |
Ts: 5′-TGGTCTCCACGAGCCTCCGAGCG-3′ | |
c-myc | Cx: 5′-GTGGCACCTCTTGAGGACCT-3′ |
Ts: 5′-TGGTGCTCCATGAGGAGACA-3′ | |
GAPDH | Cx: 5′-CGGAAGGCCATGCCT GTCAG-3′ |
Ts: 5′-GCCTCTTGCACGACCAACTG-3′ | |
Hep-G2 | the hepatoblastoma cell line |
NCI-H460 | the non-small cell lung cancer cell line |
SK-OV-3 | the human ovarian cancer cell line |
HCT-8 | the human ileocecal adenocarcinoma cell line |
BEL-7402 | the human hepatoma cell line |
HL-7702 | an human normal hepatocytes cell line |
