Figure 4 | Scientific Reports

Figure 4

From: Highly expressed ribosomal protein L34 indicates poor prognosis in osteosarcoma and its knockdown suppresses osteosarcoma proliferation probably through translational control

Figure 4

RPL34 mRNA levels in hFOB1.19 osteoblast cell line and human OS cell lines and the knockdown efficacy of RPL34 mediated by RPL34-siRNA both in mRNA and protein levels.

(A) The mRNA levels of RPL34 in hFOB1.19 cells and U2OS, MG-63, HOS and Saos-2 cell lines were detected by RT-qPCR. GAPDH was used as an internal control. (B) The fluorescence microscope was used to determine the transfection efficacy of RPL34-siRNA and NC-siRNA lentivirus. Original magnification: 100× and 200×. (C) The mRNA level of RPL34 was significantly down-regulated in RPL34-siRNA treated group compared with NC-siRNA treated group, ***P = 0.0008. (D) The lentivirus vector had no effect on the expression of RPL34 in Saos-2 cells, P = 0.091. (E) Western blot further confirmed RPL34 protein down-regulation of Saos-2 cells in RPL34-siRNA treated group, as compared to NC-siRNA treated group.

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