Figure 3
Effect of endocytosis inhibitors on GapmeR cellular internalization in human T-cells.
Primary human T-cells were untreated (A, control) or pre-treated with amiloride [0.5 mM (B), 1.0 mM (C), 2.0 mM (D), 3.0 mM (E), 4.0 mM (F) or 5.0 mM (G)], 1 μg/ml filipin (H), 10 μM chlorpromazine (I), 10 mM cytochalasin D (J) for 30 min. Cells were then incubated with 500 nM FAM-GapmeR for 24 h to allow gymnosis. Cellular internalization of GapmeR was analysed by flow cytometry. Results show dose-dependent inhibition of FAM-GapmeR cellular uptake in cells treated with amiloride (K) but not with other three inhibitors. Data represent at least three independent experiments using T-cells purified from at least 3 different donors, *p < 0.05.
