Figure 3
From: Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay
Analysis of reporter probe-AuNP conjugation using.
(a) Agarose gel image by naked eye and by UV-transilluminator, highlighting unsuccessful DNA-AuNP conjugation using MCH co-immobiliser (Lanes 2–5) or DTT as reducing agent (Lanes 6–7), whilst successful conjugation is demonstrated via the slower gel migration of DNA-AuNP conjugates without immobiliser/reducing agent (Lanes 10, 11), with the highest level of AuNP loading with DNA observed using TCEP as reducing agent (Lanes 8, 9); (b) Spectrophotometer analysis of gold nanoparticle-DNA conjugates highlighting peaks obtained at 260 nm (DNA) and 520 nm (AuNP).
