Figure 3 | Scientific Reports

Figure 3

From: Glyoxalase 1-knockdown in human aortic endothelial cells – effect on the proteome and endothelial function estimates

Figure 3

Vimentin and PLOD2.

(A) Vimentin concentration determined by ELISA (N = 4, n = 3). **p < 0.01 vs. WT25mM+NT-siRNA. $p < 0.05 vs. WT25mM. (B) mRNA Expression of vimentin in HAECs transfected with scrambled and GLO1-siRNA following 72 h siRNA transfection (N = 3, n = 2). (C) Western blot analysis performed with anti-PLOD2 in lysates from siRNA transfected HAEC (N = 4, n = 2). lane 1: Precision Plus Protein Standard; lane 2, 7: WT25mM; lane 3, 8: WT25mM+NT-siRNA; lane 4, 9: WT25mM+GLO1-siRNA; lane 5, 6: WT5mM,: *p < 0.05 vs. WT25mM+NT-siRNA. $p < 0.05 vs. WT25mM. (D) mRNA Expression of PLOD2 in HAECs transfected with NT- and GLO1-siRNA (following 72 h siRNA transfection). (N = 4, n = 2). *p < 0.05 vs. WT25mM+NT-siRNA. $p < 0.05 vs. WT25mM.

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