Figure 1

Neighbour-joining distance tree of 16S rRNA phytoplasma sequence accessions (N = 163) and sequences of nine phytoplasma-positive lucernes from Forbes, Australia.

Scale bar equals 1% equal weighted sequence difference. Cluster node supports >70% (10,000 bootstrap replicates) as indicated. Terminal 16S rRNA subgroups (refer Methods) collapsed as clusters containing multiple accessions. Tip labels indicate 16S rRNA subgroups, provisional Candidatus Phytoplasma species, and (in parentheses) associated phytoplasma strain or disease acronym. Multiple provisional species in clusters indicated as “spp.”, unknown species as “sp?”. Shaded red squares, blue squares, and blue triangles indicate phytoplasma detected in lucerne overseas, Australia and Forbes respectively. Refer Fig. 2 for greater detail of the 16SrII subgroup cluster.