Figure 6

Pneumococcal Eno mutants lacking C-terminal eRNA-binding motif do not demonstrate reduced adherence to and internalization into A549 cells.

(A,B) A549 cells were preincubated with different amounts of eRNA and infected with S. pneumoniae overexpressing wt Eno (Speno^wt) or lacking C-terminal eRNA-binding motif (Speno^int/del). In rescue experiments Speno^int/del was preincubated with soluble Eno protein (Speno^compl). Pneumococcal host-cell adherence (A) and internalization (B) were determined by immunofluorescence staining and microscopy. Data represent mean values ± SEM; n = 3; *p ≤ 0.05 vs Speno^int/del; ***p ≤ 0.001 vs untreated A549 cells.