Figure 6
From: Histone deacetylase 1 plays a predominant pro-oncogenic role in Eμ-myc driven B cell lymphoma
Hdac1 has a predominant role in non-malignant B cells.
All experiments were performed in 8-week-old animals. (A) Representative flow cytometry dot plots of B220/IgM staining, gated on B220+ lymphocytes derived from BM of mice with indicated genotypes. Gated regions in dot plots indicate B cell subsets of interest with frequency in percent: Pro/preB (B220+; IgM−), ImmatureB (B220low; IgM+), Transitional B (B220+; IgM+) and mature B (B220high; IgM+) cells. (B) Quantification of flow cytometry analysis shown in (A). Bar plots represent average numbers of cells (gated 50,000 lymphocytes) from the different B lymphocyte subsets in the BM. (C) Quantification of flow cytometry analysis shown in (A). Average numbers of absolute PreBII cells. (D) Global Hdac-activity assay performed in CD19+ MACS sorted B cells from BM of mice with indicated genotypes. Values are shown in Relative Fluorescence Units (RFU) relative to control Hdac1+/+; Hdac2+/+ cells. (E) Immunoblot analysis of Hdac1 and Hdac2 expression, and actin as loading control from CD19+ MACS sorted splenic B cells derived from mice with indicated genotypes. The cropped blots originate from a single blot for each protein. The full-length blots are presented in Supplementary Figure 7. All graphs represent mean ± s.e.m. from 3 mice of each genotype. Statistical analysis with Student unpaired 2-tailed t test, *p < 0.05; **p < 0.01. N.S., not statistically significant.
