Figure 1
From: A Role of the FUZZY ONIONS LIKE Gene in Regulating Cell Death and Defense in Arabidopsis
fzl mutations confer enhanced disease resistance to bacterial and oomycete pathogens.
(A) The FZL gene structure and fzl mutant alleles. Exons are indicated in black and introns in grey. (B) SA quantification. Total SA (glucosylated form) was extracted from the indicated plants and quantified with an HPLC instrument. (C) Expression of PR1 analyzed by qRT-PCR. (D) Bacterial growth assay. Plant leaves were infiltrated with virulent P. syringae pv. tomato strain DC3000 (OD600 = 0.0004). Leaf discs were taken 3-day post infection for bacterial growth measurement. (E) Hpa Noco2 infection assay. Seven-day-old seedlings were spray-infected with Hpa Noco 2. Sporangiophore production in cotyledons of each genotype was counted at 7 dpi. Data represent the average number of sporangiophores from 50 seedlings with SEM. Statistical analysis was performed with Student’s t-test (StatView 5.0.1). The asterisk indicates significant difference between the labeled sample and other samples (P < 0.01). These experiments were repeated at least two times and similar results were obtained.
