Figure 4
From: A Role of the FUZZY ONIONS LIKE Gene in Regulating Cell Death and Defense in Arabidopsis

Cell death conferred by fzl-Ler can be enhanced by SA treatment.
Protoplasts were prepared from 21-day old plants and treated with 100 μM BTH or water. Cell survival at the indicated times was assessed with fluorescein diacetate staining and recorded by fluorescence microscopy. The survival rate was calculated as the follow: No. of living protoplasts/no. of total protoplasts *100. Different letters indicate significant difference among the samples at the same time point (P < 0.01; Mann-Whitney test). This experiment was repeated three times and similar results were obtained.