Figure 5
From: A Role of the FUZZY ONIONS LIKE Gene in Regulating Cell Death and Defense in Arabidopsis
TEM analyses of chloroplast morphology, autophagosome, and ROS deposition.
The fourth to sixth leaves of each genotype were collected and cut into 1 × 2 mm sections, which were incubated with freshly prepared 5 mM CeCl3 in 50 mM MOPS at pH 7.2 or MOPS only for 1 hr. The samples were then fixed and processed for TEM imaging. At least three different leaf samples of each genotype were used in each experiment. Images represent typical observations in two independent experiments. (A,B) Images of Ler cells. Note that all organelles are cerium-free. (C) Image of fzl-Ler cells to show electron-dense cerium deposits mainly on the cell wall. (D–F) Images of fzl-Ler cells to show autophagosome accumulation. Arrows in panels (C,F) indicate cerium deposits. The size bar in panels (A,E,F) is 1 μm, in panel (B,D) is 5 μm, and in panel (C) is 500 nm. *autophagosome; Ch, chloroplast; CW, cell wall; and M, mitochondrion.
