Figure 4

In vivo treatment with NAC ameliorates the functional deficit of CXCR4^−/− HSPCs without influencing their proliferative status.

(a,b) CXCR4^+/+ and CXCR4^−/− chimeras were treated with NAC or vehicle, BrdU⁺ cell frequencies in BM LSK-SLAM and LSK subsets. Data represent mean ± SEM from 6 mice per group pooled from 2 independent experiments. (c) Total number of BM LSK-SLAM cells. Data represent mean ± SEM from 8 mice per group, pooled from 2 independent experiments. (d–f) Restoration of total CFC numbers after NAC-treatment in BM, spleen and peripheral blood (PB). Mean ± SEM of colonies scored for 6 samples per group of 2 independent experiments performed in duplicate.