Figure 2

Frequency dependence of D2-IPSCs evoked by dopamine terminal or ChI stimulation.

(A) Representative traces of D2-IPSCs evoked by electrical stimuli. Illustrated are IPSCs evoked by a single stimulus (black) and bursts (5 at 40 Hz, gray). (B) D2-IPSCs evoked by a single electrical stimulus are inhibited to a greater extent by DHβE (1 μM) than D2-IPSCs evoked with bursts of stimuli (5 at 40 Hz). (C) Quantification of DHβE inhibition of D2-IPSCs evoked by single or bursts of electrical stimuli (**p < 0.01, Mann-Whitney). (D) Representative traces of D2-IPSCs evoked by photostimulation of dopamine terminals. Illustrated are IPSCs evoked by a single stimulus (black) and bursts (5 at 40 Hz, gray). (E) D2-IPSCs evoked by photostimulation of dopamine terminals (DAT-Cre:ChR2) show little inhibition by DHβE (1 μM). (F) Quantification of the lack of effect of DHβE (1 μM) on D2-IPSCs evoked by photostimulation of dopamine terminals. (Mann-Whitney). (G) Representative traces of D2-IPSCs evoked by photostimulation of ChIs. Illustrated are IPSCs evoked by a single stimulus (black) and bursts (5 at 40 Hz, gray). (H) D2-IPSCs evoked by photostimulation of ChIs (ChAT-Cre:ChR2) are abolished in the presence of DHβE (1 μM). (I) Quantification of amplitude of D2-IPSCs evoked by bursts of stimulation normalized to the amplitude of single stimuli (*p < 0.05, ***p < 0.001, Wilcoxon matched-pairs signed rank within group and Mann-Whitney between groups).