Figure 7
Expression of σ1 receptors in the rostroventral medulla (RVM), periaqueductal grey matter (PAG), dorsal spinal cord (dSC) and dorsal root ganglion (DRG) after the repeated administration of PRE-084 (PRE) in capsaicin-sensitized mice and nonsensitized animals.
Mice were subcutaneously (s.c.) treated with PRE or its solvent (saline) once a day (q.d.) for 7 days. 24 h after the last s.c. injection, the mice were given an intraplantar injection of capsaicin (0.125 μg) or its vehicle (DMSO, 1%), and tissue samples were obtained 15 min later. (A) Representative immunoblots for σ1 receptors in mice after different treatments. β-actin was used as a loading control. Full-length gels are presented in Supplementary Figure 2. (B) Quantification of immunoblotting for σ1 receptors under different experimental conditions. Each bar and vertical line represents the mean ± SEM of densitometric values obtained in 4–5 animals. The result for σ1 receptor band intensities were expressed relative to those of their corresponding β-actin loading control bands. Statistically significant differences between samples from central nervous system regions (RVM, PAG, dSC) and lumbar DRGs from mice with the same treatment: **p < 0.01. There were no significant differences between mice treated with PRE or its solvent, or between animals treated with capsaicin or its vehicle, in any tissue tested (two-way ANOVA followed by Bonferroni test). All samples derive from the same experiments and gels were processed in parallel.
