Table 4 PCR primers used in this study.
Name | Site | Primer sequence (5′–3′) | Annealing temp. (°C) | Product size (bp) | CpGs (n) |
|---|---|---|---|---|---|
CpG island-1 | −1337~−1059 | F: TTTAATATTTTGGTTAATATGGGTGAAATTT | 70.4 | 279 | 14 |
R: AAAACTCCCAAAACACTCATCTATC | |||||
CpG island-2 | −163~ +76 | F: TTTATAATTTAGAGGAGGTTGGGTT | 70.1 | 250 | 12 |
R: CTACCCTCTTTTCCCACCTTAATATA | |||||
CpG island-3 | +123~ +476 | F: TTTTTGGGTTGGGAAAGAGA | 70.4 | 354 | 37 |
R: AACTACTAAACCTTAAATCAAACAAC | |||||
CpG island-4 | +630~ +953 | F: TTTAAGGTTTTGTTTGGGAGAGGATAG | 69.8 | 324 | 35 |
R: ACTAAAATCAAAAAACCAAAAAAAA | |||||
CpG island-5 | +933~ +1229 | F: TTTTGTTTTTTTGATTTTAGTTTAT | 70.0 | 297 | 20 |
R: AACCTTAATTCCCCATTTAAAATCC | |||||
VDAC2 | — | F: AGAGAAGCGGTCCCTCAGAA | 68.9 | 100 | — |
R: AAACAAAGCCCTGCATAGGGT | |||||
β-actin | — | F: TCACCCACACTGTGCCCATCTACGA | 72.3 | 295 | — |
R: CAGCGGAACCGCTCATTGCCAATGG |
