Figure 8
Expression of SPOCK-3 splice variants in pancreatic stellate cells.
(a) PS-1 cell lysates were treated with PNGase-F glycosidase and analysed by western blot using antibodies to SPOCK-3 and β actin as a loading control. Images are representative of 3 independent replicates, and the mean molecular weight observed for each band is indicated next to the blots. Arrows indicate de-glycosylated bands. Uncropped blots are shown in Suppl. Figure 5. (b) PS-1 mRNA was isolated and expression of the indicated SPOCK-3 splice variant expression was analysed by RT-PCR.
