Figure 2 | Scientific Reports

Figure 2

From: In silico clustering of Salmonella global gene expression data reveals novel genes co-regulated with the SPI-1 virulence genes through HilD

Figure 2

HilD positively regulates gtgE, phoH, sinR, lpxR, SL1896 and SL4247 in S. Typhimurium.

Expression of the gtgE-cat (A), phoH-cat (B), sinR-cat (C), lpxR-cat (D), SL1896-cat (E) and SL4247-cat (F) transcriptional fusions contained in plasmids pgtgE-cat, pphoH-cat, psinR-cat, plpxR-cat, pSL1896-cat and pSL4247-cat, respectively, was tested in the WT S. Typhimurium strain SL1344 and its isogenic ΔhilD mutant carrying the vector pMPM-K6 or the plasmid pK6-HilD, which expresses HilD under an arabinose-inducible promoter. CAT-specific activity was determined from samples collected of bacterial cultures grown for 9 h in LB medium at 37 °C. Expression of HilD from pK6-HilD was induced by adding 0.001% L-arabinose to the medium at the beginning of the bacterial cultures. The data are the average of three independent experiments performed in duplicate. Bars represent the standard deviations. *Expression statistically different with respect to that shown by the same transcriptional fusions in the ΔhilD mutant carrying the vector pMPM-K6, P-value < 0.001.

Back to article page