Figure 4 | Scientific Reports

Figure 4

From: In silico clustering of Salmonella global gene expression data reveals novel genes co-regulated with the SPI-1 virulence genes through HilD

Figure 4

HilD binds to the regulatory region of gtgE, phoH, sinR, lpxR and SL4247, but not to that of SL1896.

MBP-HilD binding to the DNA fragments contained in the gtgE-cat (A), phoH-cat (B), sinR-cat (C), lpxR-cat (D), SL4247-cat (E) and SL1896-cat (F) transcriptional fusions was analyzed by competitive nonradioactive EMSAs. As a positive control, the regulatory region of hilA was also assessed (G), and as a negative internal control, a DNA fragment containing the regulatory region of ppk or sigD was included in each DNA-binding reaction. PCR-amplified and purified DNA fragments were incubated with increasing concentrations (0 to 1 μM) of purified MBP-HilD fusion protein. The DNA-protein complexes (indicated by an asterisk) were resolved in a nondenaturing 5% polyacrylamide gel and stained with ethidium bromide.

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