Figure 2
From: Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
Schematic representation of the two investigated depletion protocols.
Beads-based depletion of 5′ tRNA halves involves the use of biotinylated DNA probes with complementarity to the 5′ tRNA half sequences. After DNA/RNA hybridization, probes are bound by magnetic streptavidin beads and immobilized using a magnetic field. Finally, the supernatant, containing purified RNA, is collected and purified by ethanol precipitation. RNase H-based depletion relies on the addition of complementary DNA probes followed by specific cleavage of RNA in DNA/RNA duplexes by RNase H. Remaining DNA probes are then degraded using DNAse I and the depleted RNA purified by ethanol precipitation. See methods section for a more detailed description.
