Figure 2 | Scientific Reports

Figure 2

From: Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy

Figure 2

Comparison of physicochemical properties between CT-TALE and VT-TALE.

(a) Analyses of size exclusion chromatograms of TALEs. Calibration of the column was performed using a standard globular protein set (GE Healthcare), and the chromatogram is presented in green. Stokes radii of the standard proteins are labelled (Å unit)27. The chromatograms of CT-TALE and VT-TALE are indicated in blue and red, respectively. Estimated Stokes radii of CT-TALE and VT-TALE are shown in parentheses. (b) CD spectra of TALEs. CD spectra of CT-TALE and VT-TALE are presented in blue and red, respectively. (c) Size distributions of CT- and VT-TALE. Particle size distributions of CT- and VT-TALE are presented in blue and red, respectively. Estimated Z-average sizes were 48.3 ± 0.7 Å and 44.7 ± 1.6 Å, respectively. (d) DSC thermograms of the CT- and VT-TALEs. Experimental data are shown in black, and fits for individual and composite fits are indicated in red. (upper) CT-TALE: T1m = 61.71 ± 0.02 °C; T2m = 63.41 ± 0.01 °C, (lower) VT-TALE: Tm = 52.07 ± 0.01 °C.

Back to article page