Figure 4 | Scientific Reports

Figure 4

From: Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy

Figure 4

Comparison between the DNA binding mechanism of VT-TALE and CT-TALE.

(a) The DNA sequences used in this study. EBEf (effector binding element): full target sequence; non-EBE: non-target sequence; EBEn: N-terminal half (5′ side) TAL-repeat array target sequence; EBEc: C-terminal half (3′ side) TAL-repeat array target sequence. The underlined nucleotides are target sequences recognised by our TALE samples. (b) ITC analyses of CT-TALE and VT-TALE against a series of DNAs. Each plot of the total heat released is presented as a function of the molar ratio of added DNA, which was subtracted by the heat generated owing to the dilution of the titrants. The red lines represent the nonlinear, least-squares best fit to the experimental data, using a one-site model. (c) The thermodynamic signatures of TALEs binding to EBEf (left) and EBEn (right). The upper and lower panels indicate the results at 15 and 25 °C, respectively. The results of CT-TALE and VT-TALE are presented in black and red, respectively. All experiments were carried out twice.

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