Table 1 ITC-derived thermodynamic parameters of the binding of TALE to DNA.

DNA^a	Temp. (°C)	TALE	Thermodynamic parameters^b
DNA^a	Temp. (°C)	TALE	Sites (n)	ΔG (kcal/mol)	ΔH (kcal/mol)	−TΔS (kcal/mol)	K_D (nM)
EBEf	15	CT-TALE	0.94 ± 0.05	−9.02 ± 0.17	−5.42 ± 0.26	−3.60 ± 0.31	138.89 ± 40.81
	15	VT-TALE	0.88 ± 0.05	−9.06 ± 0.07	−6.05 ± 0.21	−3.02 ± 0.22	131.33 ± 16.20
	25	CT-TALE	0.99 ± 0.01	−8.80 ± 0.15	−13.01 ± 0.92	4.22 ± 0.93	345.98 ± 80.94
	25	VT-TALE	0.93 ± 0.02	−8.84 ± 0.06	−15.23 ± 0.71	6.39 ± 0.71	325.73 ± 34.33
EBEn	15	CT-TALE	1.03 ± 0.03	−9.17 ± 0.09	−5.47 ± 0.38	−3.70 ± 0.39	108.90 ± 16.52
	15	VT-TALE	0.99 ± 0.04	−9.11 ± 0.21	−5.54 ± 0.42	−3.57 ± 0.47	117.03 ± 46.07
	25	CT-TALE	1.13 ± 0.03	−8.87 ± 0.17	−11.87 ± 0.49	3.00 ± 0.52	305.31 ± 88.05
	25	VT-TALE	1.12 ± 0.02	−8.97 ± 0.07	−12.66 ± 0.33	3.69 ± 0.34	261.85 ± 31.04

^aEBE represents ‘effector binding element’. EBEf contains the full recognition sequence, whereas EBEn has the sequence recognised by the N-terminal eight TAL-repeats. The other types of DNA used in this work (Fig. 4a) did not give any thermograph responses; thus, they are not listed in this table.
^bEach measurement was repeated thrice.

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