Figure 4 | Scientific Reports

Figure 4

From: A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

Figure 4

Large DNA fragment deletion in the E. coli MG1655 chromosome by CA-NHEJ.

(a) Schematic of chromosomal fragment deletions using CA-NHEJ. Four sgRNA pairs, L4&LR8, LI10&LA0, LI10&CR0, ME17&CR0, were designed for the deletion of the lacZ gene, lac operon, lac-cyn operons, and mhp-lac-cyn operons, respectively. The distribution of the sgRNA pairs and the DNA fragments expected to be deleted by separate sgRNA pairs are detailed and highlighted. Cyan: the mhp operon; tan: the lac operon; green: the cyn operon. (b) PCR analysis of six transformants from each engineering experiment, used to identify the lengths of deleted DNA fragments by various sgRNA pairs.

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