Table 1 Efficiency and positivity rate of various size chromosomal fragment deletions by CA-NHEJ.

From: A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

sgRNA pairs

Length of deleted fragments

Number of white colonies

Number of total colonies

Average positivity rate (%)

L4

~100 bp

521 ± 75

882 ± 133

59.3 ± 4.5

L4&LR8

~3 Kb

83 ± 14

112 ± 12

49.1 ± 2.1*

LI10&LA0

~6 Kb

28 ± 7

74 ± 5

36.8 ± 6.5

LI10&CR0

~10 kb

12 ± 3

51 ± 13

25.6 ± 4.0

ME17&CR0

~17 kb

4 ± 1

24 ± 3

17.3 ± 3.6

  1. *The proportion of white colonies was 75.3 ± 3.2% on the blue-white screening plate, while 65.2% (30/46) white colonies possessed complete deletion of lacZ according PCR analysis (Supplementary Fig. 6). Hence, the final positivity rate for deletion of the complete lacZ gene was corrected by deducting the false-positive colonies (65.2% × 75.3 ± 3.2%). All results were obtained from three independent trials.
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