Figure 4
Cell shape analysis between different topographies.
Panel A shows the cell shape metrics for 21 d culture of ASCs over flat, flat-osteo, random and aligned topographies. The shape metrics area, circularity, major axis length and minor axis length are taken from image analysis completed with CellProfiler. Panel B shows the relative magnitude of features (z axis) at a given angle (x axis) over the 21 d (y axis) culture period is shown. The relative magnitude of different angles shows strong and consistent alignment for aligned topographies. Panel C shows representative images of flat, flat-osteo, random and aligned topographies at 0.5, 3 and 16 day time points (scale bars 100 μm).
