Figure 4 | Scientific Reports

Figure 4

From: A-Kinase Anchoring Protein 4 (AKAP4) is an ERK1/2 substrate and a switch molecule between cAMP/PKA and PKC/ERK1/2 in human spermatozoa

Figure 4

cAMP inhibits PMA-induced ERK1/2 activation in HEK293T cells expressing proAKAP4.

HEK293T cells were transfected with turboGFP-proAKAP4 or with turboGFP. Following the transfection (48 hours) the cells were preincubated with or without 8-Br-cAMP (1 mM) for 10 min, followed by treatment with or without PMA (25 nM) for the indicated time (0–90 min). After treatment, cell lysates were analyzed for ERK1/2 activity as in Fig. 2. A representative blot is shown from three similar experiments. 8-Br-cAMP (lanes 5 and 6), had a pronounced inhibitory effect on PMA-stimulated ERK1/2 activation in the tGFP-proAKAP4 vs. the tGFP expressing cells.

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