Figure 5 | Scientific Reports

Figure 5

From: A-Kinase Anchoring Protein 4 (AKAP4) is an ERK1/2 substrate and a switch molecule between cAMP/PKA and PKC/ERK1/2 in human spermatozoa

Figure 5

Association between Raf-1 and proAKAP4.

Human spermatozoa were washed and preincubated with or without 8-Br-cAMP(1 mM) for 10 min followed by treatment with or without PMA (25 nM) for 15 min. Cells lysate were immunoprecipitated with anti-AKAP4, followed by western blotting with anti-Raf-1 or anti-AKAP4 for loading control. Beads without anti-AKAP4 antibody were incubated with cell lysates to serve as antibody specificity control (lane 5). A representative blot is shown and bars are mean ± SEM from three similar experiments. Although a decrease is observed in lanes 2, 3, note that a similar decrease is observed in the amount of the immunoprecipitated proAKAP4.

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