Figure 2
Detection of the blaCTX-M-15 gene in two samples.
(a) Consensus barcode for plasmid pEC019 cut either with light (control, gray) or with Cas9 targeting the blaCTX-M-15 gene (dark gray). (b) Histogram showing the location of the dsbreaks in the control sample (gray) and the sample treated with Cas9 targeting the blaCTX-M-15 gene (dark gray). Using the Cas9, a vast majority of the dsbreaks appear at the same location, showing that the blaCTX-M-15 gene is present. (c) Theoretical barcode for plasmid pUUH239.2 where the circles indicate the presence of the blaCTX-M-15 gene (dark gray) and the repA2 gene (light gray), respectively. (d) Histogram showing the location of the dsbreaks in the control sample (gray) and the sample treated with Cas9 targeting the blaCTX-M-15 gene (dark gray) or the repA2 gene (light gray). Using the Cas9, a vast majority of the cuts appear at the predicted locations, showing that the genes are present. The horizontal lines in the histograms correspond to the mean value for the control experiments (dashed line) and three standard deviations above the mean for all experiments (solid lines) from the balls-in-boxes statistics.
