Figure 5 | Scientific Reports

Figure 5

From: Hyperglycaemia and Pseudomonas aeruginosa acidify cystic fibrosis airway surface liquid by elevating epithelial monocarboxylate transporter 2 dependent lactate-H+ secretion

Figure 5

Hyperglycaemia induces MCT-dependent ASL acidification in airway epithelial cultures lacking CFTR-dependent bicarbonate secretion.

(A) H441 ASL pH after 6 hours in 5 or 15 mM basolateral D-glucose, in the absence or presence of 100 nM MCT2 inhibitor AR-C155858 (ARC) *P < 0.05, **P < 0.01, n = 5. (B) Calu-3 ASL pH after 6 hours in HCO3− or HEPES-buffered basolateral salt solution containing 5 or 15 mM D-glucose, in the absence or presence of 100 nM AR-C155858 (ARC) *P < 0.05, n = 9. (C) HBE or CF HBE ASL pH after 6 hours in 5 or 15 mM basolateral D-glucose, in the absence or presence of 100 nM AR-C155858 (ARC) *P < 0.05, n = 5. Data represent the means ± S.E.M.

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