Figure 6
P. aeruginosa further acidifies airway ASL and enhances bacterial growth in the absence of CFTR-dependent bicarbonate secretion.
(A) pH of HBE ASL after 6 hours in basolateral salt solution containing 5 mM D-glucose (HBE only) or in the presence of apical P. aeruginosa PA01 (+1 × 105–107 CFU), or pH of fluid from bacterial culture in the absence of HBE cells (1 × 107 CFU PA01 only) n = 6. B. CF HBE ASL pH after 6 hours in the absence (CF HBE only) and presence of 1 × 106 CFU apical PA01 (+PAO1), in basolateral HCO3−-buffered salt solution containing 5 or 15 mM D-glucose. (C) HBE ASL pH after 6 hours in the absence (open bars) or presence (black bars) of 1 × 106 CFU apical PA01 (+PA01) in basolateral HCO3− or HEPES-buffered salt solution containing 5 mM D-glucose with or without 5 μM forskolin (FSK) *P < 0.05, **P < 0.01, n = 5. D. HBE ASL pH after 6 hours in HCO3− or HEPES-buffered basolateral salt solution containing 5 or 15 mM D-glucose in the presence of 1 × 106 CFU apical PA01 *P < 0.05, **P < 0.01, n = 5. E. P. aeruginosa CFU on the apical surface of HBE monolayers after 6 hours in HCO3− or HEPES-buffered basolateral salt solution containing 5 or 15 mM D-glucose *P < 0.05, **P < 0.01, n = 5. Data represent the means ± S.E.M.
