Figure 3
From: Calmodulin interacts with Rab3D and modulates osteoclastic bone resorption
Calmodulin and Rab3D are co-expressed during osteoclast formation and co-fractionated in the membrane fraction of osteoclasts.
(A) Representative images from BMMs cultured in the present of M-CSF and RANKL for a period of 0, 1, 3, 5 days and then fixed and stained for TRACP activity. (B) Semi-quantitative RT-PCR showing the co-expression of calmodulin and Rab3D along with osteoclast marker gene expression of TRACP, NFATc1, DC-STAMP, V-ATPase d2, and calcitonin receptor. (C) Western blot analysis showing that calmodulin and Rab3D proteins are expressed at similar kinetics during osteoclastogenesis. (D) Sucrose gradient sedimentation assays showing that Rab3D and calmodulin co-fractionate in the large membrane faction (P = Pellet). Note that all Western blot results with cropped blots are displayed from well established sizes of proteins; calmodulin, Rab3D and VATPase (d2).
