Figure 3

Cellular uptake of PKH67-labelled viruses by hCMEC/D3 (a,b) and HUVECs (c,d) cells. Left panel: Average fluorescence intensity of cells after exposure to increasing concentration of labeled viruses for 24 h (a,c); Right panel: Quantification of positive cells bearing PKH67-labelled viruses by FACS (b,d). In all the experiments, corresponding cells were incubated with different concentration of either SMV1-PKH67 or SSV2-PKH67 for 24 h. Viral concentrations were estimated by NTA.