Figure 4

Dendritic length characteristics may be stratified according to tufted or slender neuronal types, however spine density characteristics are only affected by genotype.

WT (not hashed) and TDP-43^Q331K (hashed) LVPNs were classified into tufted and slender pyramidal neurons. Compared to tufted LVPNs, slender LVPNS are significantly reduced in length in their apical terminal length (a), total arbor length (b) and apical arbor length (c), with no significant difference between WT controls and TDP-43^Q331K LVPNs of either type. There was no difference based on neuronal type or genotype in the mean basal tree length of LVPNs (d). Compared to tufted LVPNs, slender LVPNS are significantly reduced in the number of apical bifurcation nodes and dendritic endings (e), with no significant difference between WT controls and TDP-43^Q331K LVPNs of either type. Compared to TDP-43^Q331K LVPNs, WT LVPNs had reduced total spine density (f), apical spine density (g), apical distal spine density (h) and basal spine density (i), with no difference between tufted and slender neuronal types. All data represented as mean ± s.e.m., two-way ANOVAs (tufted: n = 7 WT and TDP-43^Q331K; slender: WT n = 5 WT, n = 4 TDP-43^Q331K), *P < 0.05, **P < 0.01, ***P < 0.001.