Figure 2 | Scientific Reports

Figure 2

From: Pharmacologic modulation of RORγt translates to efficacy in preclinical and translational models of psoriasis and inflammatory arthritis

Figure 2

JNJ-54271074 blocked mouse Th17 differentiation and IL-17A production in vitro.

Pooled purified naive CD4+ T cells from spleens of four C57BL/6 mice were cultured under Th17-polarizing condition for 3 days in the absence (DMSO control) or presence of titrated JNJ-54271074 (starting at 1 μM and 5-fold serial dilution in duplicates). (A) Accumulated IL-17A and TNFα in cell culture supernatants. Representative data from one of 3 experiments are presented as mean ± SEM of duplicate samples. (B) Flow cytometry analysis of IL-17A-, TNFα- and IFNγ-producing CD4+ T cells for DMSO and JNJ-54271074 at 0.2 μM and 1 μM. Pooled duplicate samples were used for analysis. (C) Dose response curve of JNJ-54271074 on IL-17A- and TNFα-producing CD4+ T cells.

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