Figure 5
JNJ-54271074 attenuated the development of collagen-induced arthritis (CIA) in mice.
(A) Time course of CIA clinical score following treatment with vehicle or JNJ-54271074 (0.3, 3, 10, 30 and 60 mpk BID). Clinical scores were monitored on day 21, 28, 31 and 34 after the immunization (n = 8-10 mice per group). Values are the mean ± SEM. **p < 0.01, ***p < 0.005 versus vehicle by one way ANOVA (Dunnett’s multiple comparisons test). (B) Total histopathology score of four pathology indices (inflammation, cartilage damage, bone destruction and remodeling) in hind paws, harvested on day 35 (n = 14-18 paws per group). Values are the mean ± SEM. *p < 0.05 versus vehicle by one way ANOVA (Bonferroni’s Multiple Comparison Test). (C) Flow cytometry analysis on CD3+ T cell population in paws on day 35. Front paws were digested, and cells were harvested and pooled for staining. (D) Flow cytometry analysis of CD3+ T cells in front paws for IL-17A- and IFNγ- producing cells. Both FACS plots are representatives from one mouse and the % IL-17A-producing cells are also shown in histogram as mean ± SD of four mice. The results are representative of three independent experiments. The statistical analyses were performed using one way ANOVA Dunnett’s test, *P < 0.05; **P < 0.01; ***P < 0.005.
