Figure 8

Streptococcal extract (SE)-induced cytokine production in the co-culture of CLA⁺ or CLA⁻ T cells with autologous epidermal cells from guttate psoriasis patients.

The cells were cultured for 5 days in the absence or presence of 1 μM JNJ-54271074. Accumulated cytokines (IL-17A, IL-17F, IFNγ, IL-6, IL-8 and TNFα) in supernatants were quantified by multiplex fluorescent bead-based immunoassay. Data are pooled and presented as mean values ± SD of five independent experiments, one patient per experiment. Statistical analyses were performed using unpaired t test with Welch’s correction, *P < 0.05; **P < 0.01; ***P < 0.005.