Figure 2 | Scientific Reports

Figure 2

From: Biochemical and Structural Analysis of a Novel Esterase from Caulobacter crescentus related to Penicillin-Binding Protein (PBP)

Figure 2

Characterization of CcEstA.

(A) Purified CcEstA was analyzed by SDS-PAGE and then stained with Coomassie Brilliant Blue (left; molecular standards marker, right; purified CcEstA). (B) Native-PAGE analysis with 4-MU overlay assay of CcEstA. Only one band appears on Native-PAGE gel (left) showing the fluorescent signal from 4-MU catalysis (right). (C) Size exclusion chromatography of CcEstA under non-denaturing conditions. (D) MALDI-TOF mass spectrum of CcEstA. The [M+H]+ and [M+2 H]2+ ion peaks were found at m/z value of 20,552, and 41,179, respectively.

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