Figure 3

Enzyme assays of CcEstA.

(A) Substrate-specificity assay of CcEstA using p-nitrophenyl esters with different acyl chain lengths. (B) Regioselectivity assay of CcEstA using naphthyl derivatives. (C) Activity of CcEstA in various temperatures from 20 to 60 °C for 1 h. (D) Activity of CcEstA in different pH solutions from pH 3 to 10.