Figure 4 | Scientific Reports

Figure 4

From: Biochemical and Structural Analysis of a Novel Esterase from Caulobacter crescentus related to Penicillin-Binding Protein (PBP)

Figure 4

Structural stability of CcEstA.

(A,B) Thermal analysis of CcEstA was monitored in various temperature from 20 to 60 °C using circular dichroism spectrometry (A; from 190 to 260 nm, B; at 222 nm). Note that CcEstA undergoes notable changes in structure above 50 °C. (C,D) Chemical unfolding of CcEstA. Intrinsic fluorescence spectra (C) and relative enzyme activity of CcEstA with various concentrations of urea from 0 to 5 M were recorded after 1 h incubation (D).

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