Figure 3
Infected animals are only temporarily able to control S. aureus dependent disease.
(A–C) Log10 CFU counts recovered from the knee joints (CFU/organ, A), kidneys (B, CFU/organ) and blood (CFU/ml, C) of mice intravenously infected with S. aureus Newman strain from day 3 to day 90 p.i. Medians and interquartile ranges of 5 to15 animals/time point from 2/3 independent experiments are shown. ldv = lower detectable value. (D) Survival curve of mice intravenously infected with Newman strain. (E,F) IgM and IgG titration against S. aureus alpha-hemolysinH35L (HlaH35L) in sera (E) and knee joint washes (F) of infected mice from day 0 (naïve animals) to day 90. Graphs report medians and interquartile ranges of Mean Fluorescence Intensity (MFI) signals of 6 to 8 mice at each time point and 2 independent experiments. In all the graphs reported above, the vertical dotted grey lines indicate the edges of the 3 proposed stages of infection.
