Figure 2
From: Tumor-specific delivery of biologics by a novel T-cell line HOZOT
HOZOT cell–based delivery of OBP-401/F35 into human tumor cells.
(a) Schematic diagram of the OBP-401/F35 structure. OBP-401/F35 is a telomerase-specific, replication-competent, adenovirus serotype 35 fiber–modified adenovirus serotype 5, in which the hTERT promoter drives the expression of the IRES-linked E1A and E1B genes and the cytomegalovirus (CMV)promoter drives the expression of the GFP gene inserted in the E3 region. (b) Phase-contrast and fluorescence images of HOZOT cells infected with OBP-401/F35. Scale bar: 100 μm. (c) Phase-contrast and fluorescence images of HCT116-RFP cells after treatment with virus-loaded HOZOT-401/F35 cells. White arrowheads indicate HOZOT cells that penetrated HCT116-RFP cells. Scale bars: 20 μm. (d) Phase-contrast and fluorescence images of HCT116-RFP cells after treatment with mock, virus-free HOZOT cells, or virus-loaded HOZOT-401/F35 cells. Scale bars: 200 μm.
